software

In scientific experiments it is often important to conduct an experiment in
triplicate. I find that I often need to plot these on a bar chart eg cell
number with two growth conditions at a variety of different time points. If
you count the cell number in three separate flasks for each growth condition
at each time point, then you can work out the mean cell number and the SEM or
standard deviation. I want to plot both the mean and the SD for each point by
using error bars. Obviously the SD is different for each point. Excel will
not let you do this and it makes me crazy because I have to plot cell counts
for each time point and each condition as a separate series. Why is there no
quot;vary error bar by pointquot; command like there is for colour???????

Does anyone know of an easier way please?

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www.microsoft.com/office/comm...excel.charting

It does allow the specification of error bars by point.

Calculate the error bar values in some column. Then, double-click the
series in the chart and in the Format Data Series dialog box select the Y
Error Bars tab (or the X Error bars tab if appropriate). In there, select
the Custom option and specify the range containing the and/or - error
values.--
Regards,

Tushar Mehta
www.tushar-mehta.com
Excel, PowerPoint, and VBA add-ins, tutorials
Custom MS Office productivity solutions

In article gt;,says...
gt; In scientific experiments it is often important to conduct an experiment in
gt; triplicate. I find that I often need to plot these on a bar chart eg cell
gt; number with two growth conditions at a variety of different time points. If
gt; you count the cell number in three separate flasks for each growth condition
gt; at each time point, then you can work out the mean cell number and the SEM or
gt; standard deviation. I want to plot both the mean and the SD for each point by
gt; using error bars. Obviously the SD is different for each point. Excel will
gt; not let you do this and it makes me crazy because I have to plot cell counts
gt; for each time point and each condition as a separate series. Why is there no
gt; quot;vary error bar by pointquot; command like there is for colour???????
gt;
gt; Does anyone know of an easier way please?
gt;
gt; ----------------
gt; This post is a suggestion for Microsoft, and Microsoft responds to the
gt; suggestions with the most votes. To vote for this suggestion, click the quot;I
gt; Agreequot; button in the message pane. If you do not see the button, follow this
gt; link to open the suggestion in the Microsoft Web-based Newsreader and then
gt; click quot;I Agreequot; in the message pane.
gt;
gt; www.microsoft.com/office/comm...excel.charting
gt;


I'm interested in the same query.

I've calculated the standard error margin (SEM) for each point in my
bar graph - this varies as each point is the average of a varying
number of samples.

This SEM value is represented in the same units as the data on the bar
graph (second), and is in a column that corresponds to the values for
the bars in the bar graph. (i.e. A1 = Bar 1 value, B1 = Bar 1 value
SEM).

When I define the error bar using the 'custom error bars' option in the
'add error bars' tab I select the column (I drag from the top of the SEM
data to the bottom) for the SEM data. However, Excel gives seemingly
spurious error bars when I do this. I'm getting errors of several times
the values according to the error bars, when the actual SEM is equal to
zero, for example.

How do I define the error bars correctly? Is because I am working in
seconds?

I have attached the sheet. The values of the bar chart are in column
AU, and the values of each point's SEM is in column BB.

(You'll also notice the ridiculous formula I came up with for
calculating the SEM! I'm not very good with Excel!)

Thanks for your help,

Adam--
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